Using magnetotelluric (MT) sounding technology, combined with the geological and physical characteristics of the survey area, the authors made a ground-underground comparative analysis of the fractures, folds and strata in middle and upper Yangtze region. The results show that the divisions of the structures are basically consistent with the distribution of surface structures. Integrated stratigraphic distribution, resistivity anomalies and geological conditions were used to explain the characteristics of the local structure. According to the local structural features and stratigraphic sequence, combined with the oil and gas geological analysis, the authors preliminarily hold that the electric characteristics of local structure in the western and central parts of the profile is clear, and that Cambrian and Sinian strata are buried under 4 000 m, the Silurian and Ordovician low resistivity beds are stably distributed, the capping beds are extensively developed, and the faults have relatively plugging properties, which are favorable for the preservation of Cambrian and Sinian oil and gas.