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Characteristics of bioavailable selenium in different types of selenium-rich soils |
YAO Ling-Yang1,2( ), XIE Shu-Yun1,3( ), BAO Zheng-Yu1,2, MA Ming1, WAN Neng3,4 |
1. Zhejiang Institute, China University of Geosciences (Wuhan), Hangzhou 311300, China 2. Faculty of Materials Science and Chemistry, China University of Geosciences (Wuhan), Wuhan 430074, China 3. School of Earth Sciences, China University of Geosciences (Wuhan), Wuhan 430074, China 4. Wuhan Natural Resources and Planning Bureau, Wuhan 430074, China |
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Abstract Bioavailable selenium (Se) is an important indicator used to evaluate the supply capacity of Se in the soil to plants. There is a lack of national standard methods for testing bioavailable Se in the soil in China presently. This study used ten different chemical extractants to extract bioavailable Se from the soil. The results show that the extraction capacity of extractants was in the order of NaOH (10.57%) > SOL-Se+EX-Se (8.10%) > AB-DTPA (6.79) > NaHCO3 (4.99%) > K2SO4 (4.57%) > KH2PO4-K2HPO4 (4.33%) > KH2PO4 (3.05%) > SOL-Se (2.28%) > EDTA (1.40%) > NH4F-HCl (1.22%). Among them, the bioavailable Se extracted from the soil by KH2PO4, NaHCO3, SOL-Se, KH2PO4-K2HPO4, and AB-DTPA was significantly correlated with the Se content of crops. As revealed by the relationship between the percentage content of bioavailable Se in soil and soil properties, the percentage content of bioavailable Se in soil significantly correlated with indicators such as the chemical index of alteration (CIA), LOI, pH, Eh, P, and SiO2. Among them, pH and Eh mainly affect the existence form of Se in the soil, while indicators such as CIA, LOI, P, and SiO2 reflect the alteration degree of soil and the effect of adsorbates on bioavailable Se in soil. The results of this study can provide data support and a scientific basis for the development of Se-based ecological agriculture and the development and utilization of Se-rich land resources.
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Received: 07 December 2021
Published: 24 February 2023
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Corresponding Authors:
XIE Shu-Yun
E-mail: qyangw@outlook.com;tinaxie@edu.cug.com
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样品 编号 | 土壤 总硒 | C | H | N | S | TOC | SiO2 | Al2O3 | Fe2O3 | K2O | Na2O | CaO | MgO | pH | Eh | 玉米硒 | 1 | 0.70 | 1.79 | 0.606 | 0.180 | 0.127 | 1.656 | 62.042 | 14.816 | 7.758 | 2.146 | 0.928 | 1.456 | 2.202 | 5.51 | 98 | 0.122 | 2 | 0.48 | 1.02 | 0.460 | 0.100 | 0.113 | 0.852 | 66.481 | 14.698 | 5.965 | 2.620 | 0.775 | 0.514 | 1.688 | 5.94 | 77 | 0.140 | 3 | 0.37 | 1.17 | 0.428 | 0.110 | 0.119 | 1.037 | 70.545 | 12.77 | 5.371 | 2.352 | 0.748 | 0.389 | 1.334 | 5.70 | 89 | 0.039 | 4 | 0.70 | 1.73 | 0.638 | 0.220 | 0.113 | 1.505 | 62.388 | 14.819 | 5.623 | 2.496 | 0.469 | 1.716 | 3.781 | 8.17 | -29 | 0.281 | 5 | 0.75 | 1.76 | 0.716 | 0.340 | 0.107 | 1.510 | 61.497 | 15.067 | 5.780 | 2.549 | 0.471 | 1.774 | 4.260 | 8.39 | -40 | 0.464 | 6 | 0.93 | 1.90 | 0.700 | 0.360 | 0.083 | 1.711 | 59.494 | 14.898 | 6.036 | 2.461 | 0.524 | 2.995 | 4.035 | 8.26 | -34 | 0.318 | 7 | 19.54 | 3.13 | 0.482 | 0.470 | 0.062 | 2.978 | 53.873 | 13.533 | 5.263 | 2.227 | 0.352 | 7.427 | 4.826 | 8.39 | -40 | 0.493 | 8 | 14.90 | 2.65 | 0.612 | 0.590 | 0.059 | 2.402 | 55.429 | 14.054 | 6.144 | 2.277 | 0.394 | 5.412 | 4.146 | 8.07 | -24 | 16.083 | 9 | 12.68 | 1.39 | 0.686 | 0.670 | 0.054 | 1.208 | 60.004 | 15.193 | 6.472 | 2.537 | 0.482 | 2.580 | 2.623 | 8.31 | -36 | 0.215 | 10 | 9.70 | 1.58 | 0.585 | 0.140 | 0.382 | 1.263 | 58.148 | 14.546 | 6.195 | 2.642 | 0.657 | 3.818 | 3.271 | 8.14 | -28 | 11.490 | 11 | 5.64 | 1.54 | 0.528 | 0.170 | 0.262 | 1.277 | 59.893 | 15.355 | 5.848 | 3.269 | 0.627 | 2.290 | 2.901 | 8.03 | -23 | 6.970 | 12 | 4.88 | 1.68 | 0.479 | 0.170 | 0.248 | 1.460 | 60.681 | 14.76 | 5.659 | 3.255 | 0.708 | 2.444 | 2.763 | 8.53 | -47 | 6.320 | 13 | 10.53 | 1.71 | 0.518 | 0.260 | 0.168 | 1.458 | 59.96 | 13.858 | 6.188 | 2.390 | 0.374 | 2.957 | 3.420 | 8.19 | -30 | 18.420 | 14 | 12.60 | 1.51 | 0.496 | 0.240 | 0.131 | 1.321 | 58.851 | 14.040 | 6.282 | 2.412 | 0.382 | 3.382 | 3.400 | 8.08 | -25 | 16.880 | 15 | 11.94 | 1.67 | 0.528 | 0.260 | 0.141 | 1.493 | 58.746 | 14.486 | 6.230 | 2.448 | 0.374 | 2.976 | 3.563 | 8.28 | -35 | 16.630 | 16 | 16.85 | 1.23 | 0.461 | 0.200 | 0.128 | 0.933 | 57.793 | 14.535 | 6.977 | 2.580 | 0.434 | 3.319 | 3.275 | 8.13 | -27 | 12.030 | 17 | 10.48 | 1.39 | 0.500 | 0.240 | 0.096 | 1.160 | 59.148 | 14.381 | 6.297 | 2.481 | 0.406 | 3.009 | 3.305 | 7.95 | -19 | 11.490 |
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Soil physical and chemical properties and corresponding corn selenium content in Ziyang area
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编号 | 玉米总Se | 土壤总Se | 有效硒/10-6 | 10-6 | 10-6 | KH2PO4 | NaHCO3 | NaOH | KH2PO4- K2HPO4 | NH4F-HCl | EDTA | AB-DTPA | K2SO4 | SOL-Se+EX-Se | SOL-Se | 1 | 0.122 | 0.70 | 17.31 | 49.12 | 188.80 | 42.82 | 14.38 | 24.49 | 81.67 | 54.43 | 107.61 | 42.63 | 2 | 0.14 | 0.48 | 18.34 | 57.19 | 140.10 | 38.53 | 10.83 | 19.24 | 78.18 | 55.59 | 62.69 | 16.52 | 3 | 0.039 | 0.37 | 9.87 | 32.01 | 132.40 | 26.70 | 8.90 | 14.51 | 70.10 | 58.64 | 52.94 | 14.38 | 4 | 0.281 | 0.70 | 24.70 | 50.98 | 89.50 | 34.52 | 15.52 | 14.93 | 71.49 | 73.73 | 82.23 | 24.13 | 5 | 0.464 | 0.75 | 29.33 | 49.88 | 100.60 | 36.56 | 18.00 | 15.47 | 69.14 | 80.13 | 89.92 | 29.78 | 6 | 0.318 | 0.93 | 33.59 | 56.03 | 104.70 | 47.14 | 23.17 | 19.96 | 84.96 | 73.64 | 114.70 | 39.89 | 7 | 0.493 | 19.54 | 100.36 | 85.43 | 135.45 | 99.98 | 40.75 | 19.91 | 165.42 | 117.07 | 434.44 | 41.53 | 8 | 16.08 | 14.90 | 1049.00 | 1033.00 | 1064.00 | 1091.00 | 171.30 | 49.48 | 458.31 | 417.01 | 1248.00 | 223.18 | 9 | 3.15 | 12.68 | 214.35 | 212.17 | 299.60 | 246.33 | 43.69 | 26.25 | 310.89 | 123.63 | 389.67 | 149.14 | 10 | 11.49 | 9.70 | 435.83 | 497.70 | 627.62 | 492.00 | 76.02 | 67.96 | 572.23 | 160.10 | 1086.77 | 126.37 | 11 | 6.97 | 5.64 | 264.16 | 328.07 | 387.77 | 328.20 | 67.97 | 67.96 | 379.85 | 109.91 | 531.53 | 103.38 | 12 | 2.72 | 4.88 | 221.87 | 288.12 | 630.25 | 287.88 | 62.06 | 63.96 | 356.21 | 115.98 | 458.10 | 93.51 | 13 | 18.42 | 10.53 | 255.74 | 327.74 | 428.26 | 304.06 | 50.00 | 51.96 | 401.76 | 109.89 | 316.84 | 115.94 | 14 | 16.88 | 12.60 | 241.95 | 285.77 | 208.00 | 271.78 | 38.01 | 44.00 | 182.07 | 27.99 | 431.00 | 110.00 | 15 | 16.63 | 11.94 | 226.00 | 292.00 | 401.76 | 292.00 | 10.58 | 13.99 | 367.78 | 53.98 | 322.97 | 119.99 | 16 | 12.03 | 16.85 | 181.96 | 236.05 | 288.00 | 217.96 | 66.00 | 79.92 | 336.07 | 89.95 | 303.97 | 101.99 | 17 | 11.49 | 10.48 | 166.10 | 219.96 | 321.81 | 210.00 | 65.93 | 78.02 | 340.14 | 82.02 | 450.04 | 108.01 |
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Different chemical extractants extract the available selenium content in soil
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编号 | KH2PO4 | NaHCO3 | NaOH | KH2PO4- K2HPO4 | NH4F-HCl | EDTA | AB-DTPA | K2SO4 | SOL-Se+EX-Se | SOL-Se | 1 | 2.47 | 7.02 | 26.97 | 6.12 | 2.05 | 3.50 | 11.67 | 7.78 | 15.37 | 6.09 | 2 | 3.82 | 11.91 | 29.19 | 8.03 | 2.26 | 4.01 | 16.29 | 11.58 | 13.06 | 3.44 | 3 | 2.67 | 8.65 | 35.78 | 7.22 | 2.41 | 3.92 | 18.95 | 15.85 | 14.31 | 3.89 | 4 | 3.53 | 7.28 | 12.79 | 4.93 | 2.22 | 2.13 | 10.21 | 10.53 | 11.75 | 3.45 | 5 | 3.91 | 6.65 | 13.41 | 4.87 | 2.40 | 2.06 | 9.22 | 10.68 | 11.99 | 3.97 | 6 | 3.61 | 6.02 | 11.26 | 5.07 | 2.49 | 2.15 | 9.14 | 7.92 | 12.33 | 4.29 | 7 | 0.51 | 0.44 | 0.69 | 0.51 | 0.21 | 0.10 | 0.85 | 0.60 | 2.22 | 0.21 | 8 | 7.04 | 6.93 | 7.14 | 7.32 | 1.15 | 0.33 | 3.08 | 2.80 | 8.37 | 1.50 | 9 | 1.69 | 1.67 | 2.36 | 1.94 | 0.34 | 0.21 | 2.45 | 0.98 | 3.07 | 1.18 | 10 | 4.49 | 5.13 | 6.47 | 5.07 | 0.78 | 0.70 | 5.90 | 1.65 | 11.20 | 1.30 | 11 | 4.68 | 5.82 | 6.88 | 5.82 | 1.21 | 1.20 | 6.73 | 1.95 | 9.42 | 1.83 | 12 | 4.55 | 5.90 | 12.91 | 5.90 | 1.27 | 1.31 | 7.30 | 2.38 | 9.39 | 1.92 | 13 | 2.43 | 3.11 | 4.07 | 2.89 | 0.47 | 0.49 | 3.82 | 1.04 | 3.01 | 1.10 | 14 | 1.92 | 2.27 | 1.65 | 2.16 | 0.30 | 0.35 | 1.45 | 0.22 | 3.42 | 0.87 | 15 | 1.89 | 2.45 | 3.36 | 2.45 | 0.09 | 0.12 | 3.08 | 0.45 | 2.70 | 1.00 | 16 | 1.08 | 1.40 | 1.71 | 1.29 | 0.39 | 0.47 | 1.99 | 0.53 | 1.80 | 0.61 | 17 | 1.58 | 2.10 | 3.07 | 2.00 | 0.63 | 0.74 | 3.25 | 0.78 | 4.29 | 1.03 | 最小值 | 0.51 | 0.44 | 0.69 | 0.51 | 0.09 | 0.10 | 0.85 | 0.22 | 1.80 | 0.21 | 最大值 | 7.04 | 11.91 | 35.78 | 8.03 | 2.49 | 4.01 | 18.95 | 15.85 | 15.37 | 6.09 | 均值 | 3.05 | 4.99 | 10.57 | 4.33 | 1.22 | 1.40 | 6.79 | 4.57 | 8.10 | 2.22 |
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The effective selenium extracted by different chemical extractants accounts for the percentage of total selenium in the soil%
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浸提剂 | r | p | | 浸提剂 | r | p | KH2PO4 | 0.929 | 0.000 | | SOL-Se+EX-Se | 0.840 | 0.000 | NaHCO3 | 0.925 | 0.000 | | NaOH | 0.760 | 0.000 | SOL-Se | 0.922 | 0.000 | | EDTA | 0.715 | 0.001 | KH2PO4-K2HPO4 | 0.915 | 0.000 | | NH4F-HCl | 0.707 | 0.002 | AB-DTPA | 0.892 | 0.000 | | K2SO4 | 0.322 | 0.207 |
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Correlation analysis between available selenium extracted by different chemical extractants and plant selenium(n=17)
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Scatter diagram of available selenium extracted by different chemical extractants and plant selenium
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指标 | 相关系数 | | 指标 | 相关系数 | K2O | -0.434** | | Cr | -0.383** | Na2O | -0.349** | | Ni | -0.672** | CaO | -0.569** | | Cu | -0.654** | MgO | -0.593** | | Pb | -0.174 | CIA | 0.532** | | Zn | -0.614** | LOI | -0.347** | | SiO2 | 0.337* | TOC | 0.012 | | Al2O3 | -0.146 | pH | -0.622** | | Al2O3/SiO2 | -0.081 | Eh | 0.606** | | Fe2O3 | 0.002 | N | -0.400** | | MnO | -0.158 | P | -0.645** | | Fe2O3+MnO | 0.001 | S | -0.141 | | | |
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Correlation analysis between the percentage of soil available selenium and soil physical and chemical properties (n=51)
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Scatter plot of soil available selenium content and soil physical and chemical properties
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