As one of the major methods for oil and gas geochemical exploration, the hydrocarbon acidolysis technique has acquired obvious results in oil and gas exploration. Nevertheless, the application results of this method are affected by quite a few factors. Employing the mathematical statistic method to study interference factors and interference-suppressing means, the authors have made some achievements. Using the hydrocarbon acidolysis indices after interference suppression, we can classify characteristics of regional geochemical fields of major oil-and gas-bearing basins in China, predict and recognize oil and gas accumulations and reservoirs, and reveal vertical micro-migration of hydrocarbons.