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| A study of gas hydrate light hydrocarbon microseepage microbial communities and response characteristics of hydrocarbon oxidizing microorganism in Muli area |
| CHU Hou-Juan, HAO Chun, DENG Shi-Cai, MA Ting-Ting, MEI Hai |
| Beijing Angyitai Geological Microorganism Technology Co.,Ltd., Beijing 102200,China |
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Abstract Next generation sequencing was used in the study of near-surface microbial communities located in gas hydrate well DK1, DK9 and dry well DK6, Muli permafrost zone, to discover the influence of light hydrocarbon microseepage on near-surface microbial community. Compared with dry well DK6, microbial communities in gas hydrate wells changed, especially the hydrocarbon oxidizing microorganisms showed more abundance in gas hydrate well. Furthermore, the predominant hydrocarbon oxidizing microorganisms in gas hydrate well DK1 were alkane oxidizing, and those in well DK9 were methane oxidizing, in accordance with the different gas components in the gas hydrate wells. On the other hand, there were no predominant oxidizing microorganisms in dry well DK6. Besides, selective culture using butanol as the only carbon source also got a number of popular hydrocarbon oxidizing microorganisms, which could be used as the indicators of light hydrocarbon microseepage related to gas hydrate. Above all, the microbial communities, especially both uncultured and cultured hydrocarbon oxidizing microorganisms, showed detectable response to the gas hydrate underground, confirming the feasibility of microseepage based microbial exploration technology used in Muli permafrost gas hydrate survey.
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Received: 08 September 2017
Published: 20 December 2017
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