It is of great importance to study and identify geochemical anomalies caused by some interfering factors and find out the eliminating methods in developing and applying comprehensive oil and gas geochemical exploration techniques so as to recognize the real anomaly information associated with oil and gas activities. Four interfering factors have been preliminarily summarized as follows: ①interference of surface special landscape; ②interference caused by soil characteristics such as clay minerals in soil, chemical compositions and soil acidity; ③underground non-oil and gas sources like faults, high carbon matter or coal layer and rocks; ④other factors, such as pollution, river flow or atmospheric influence. Characteristics of interfering anomalies and their eliminating method are also discussed.