Abstract: As mature oilfields are in their middle to late exploitation stage, the Gaotaizi oil layer exhibits significantly increased water saturation, elusive longitudinal stratification, and unknown distribution patterns of regional residual oil. These complicate the potential exploitation, energy conservation, and emission reduction. To ascertain the distribution of residual oil in the Gaotaizi oil layer in order to guide production operations and clarify potential targets, this study identified the longitudinal distribution of residual oil in the study area using the pulsed neutron-neutron (PNN) logging technique. The comparison between the monitoring results and production data of 10 wells revealed a coincidence rate of measures of 80%. The results of this study show that the PNN logging technique can be used to effectively identify the longitudinal distribution of residual oil in reservoirs and yields encouraging application performance in guiding the hole filling and water plugging for single wells in high-water-cut layers, thus increasing oil production. This technique has increased the evaluation accuracy of residual oil saturation in the Gaotaizi oil layer in the mature oilfield. It can provide technical support for formulating and adjusting subsequent oilfield exploitation schemes and offer a scientific basis for further guiding efficient reservoir exploitation, energy conservation, and emission reduction.