The Shuihekou gold deposit in Funing of Yunnan Province is a part of the Southeast Yunnan micro-fine-disseminated gold metallogenic belt. Soil geochemistry is a simple and effective method in search for micro-fine-disseminated gold deposits. Based on the 1:10000 soil geochemical survey in the study area, the authors analyzed the content and distribution characteristics of the four elements of Au, Hg, Sb, As and delineated the anomaly. The anomaly was evaluated in combiantion with the geological features of layer, structure, lithology and the prospecting indicators of Southeast Yunnan micro-fine-disseminated gold metallogenic belt, thus delineating six prospecting target areas. An orebody was found through engineering verification of No. 3 prospecting target, which provides effective evidence for prospecting potential of the other areas in The Shuihekou gold deposit.