Compared with traditional culture-dependent methods, culture-independent techniques based on functional genes for detecting oil and gas indicating microorganisms are more rapid, stable and specific, thereby suitable for commercial applications. In this study, the authors chose Chen 22 wells zone in Shaojia subsag of Zhanhua SAG as a model system. Based on the analyses of the soil samples collected from point, linear and areal surveys, the authors detected that the anomalies of gene abundance were perfectly consistent with the presence of the oil and gas reservoirs. Therefore, microbial molecular prospecting technique for oil and gas is very promising in the future.
[1] Miqueletto P B, Andreote F D, Dias A C, et al.Cultivation-independent methods applied to the microbial prospection of oil and gas in soil from a sedimentary basin in Brazil[J].AMB Express, 2011, 1:35.[2] 程楷. 油气微生物勘探解释模型的初步实现[D].上海:华东师范大学, 2009.[3] 茆震,梅博文,赵红静.油气微生物勘探法概述[J].海相油气地质,2000,5(3/4):107-111.[4] 田建华,苏朝光.陈家庄凸起西段陈22块东营组地层超覆油藏地震描述技术[J].油气地质与采收率,2008,15(3):50-53.[5] 梅博文,袁志华.地质微生物技术在油气勘探开发中的应用[J].天然气地球科学,2004,15(2): 156-161.[6] 滕小兰,译.石油勘探和油藏描述的一种潜在新工具——微生物DNA探针应用[J].能源科学进展,2007,3(2):12-16.[7] 张春林, 庞雄奇, 梅海, 等.微生物油气勘探技术的实践与发展[J].新疆石油地质, 2010, 31 (3): 320-322.[8] 张春林, 庞雄奇, 梅海, 等.微生物油气勘探技术在岩性气藏勘探中的应用——以柴达木盆地三湖坳陷为例[J].石油勘探与开发, 2010, 37 (3): 310-315.[9] 索孝东, 石东阳.油气地球化学勘探技术发展现状与方向[J].天然气地球科学, 2008, 19 (2): 286-292.[10] 张春林, 庞雄奇, 梅海, 等.烃类微渗漏与宏渗漏的识别及镇巴长岭—龙王沟地区勘探实践[J].天然气地球科学, 2009, 20 (5): 794-800.[11] 李勇梅, 袁志华.阳信洼陷石油微生物勘探研究[J].特种油气藏, 2009, 16 (5): 44-47.[12] 苏新, 陈芳, 张勇, 等.海洋天然气水合物勘查和识别新技术:地质微生物技术[J].现代地质, 2010, 24 (3): 409-423.[13] 孔淑琼, 黄晓武, 李斌.天然气库土壤中细菌及甲烷氧化菌的数量分布特性研究[J].长江大学学报, 2009, 6: 56-59.[14] De W R,Bouvier T. 'Everything is everywhere, but, the environment selects'; what did baas becking and beijerinck really say?[J].Environmental Microbiology, 2006, 8: 755-758.[15] Yun J, Zhuang G, Ma A, et al. Community structure, abundance and activity of methanotrophs in the Zoige Wetland of the Tibetan Plateau[J].Microbial Ecology, 2012, 63: 835-843.[16] Redmond M C, Valentine D L, Sessions A L.Identification of novel methane-, ethane-, and propane-oxidizing bacteria at marine hydrocarbon seeps by stable isotope probing[J].Appl Environ Microbiol, 2010, 76: 6412-6422.[17] Zhang F, She Y, Zheng Y, et al.Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China[J].Appl Microbiol Biotechnol, 2010, 86: 1183-1194.[18] Yan T, Ye Q, Zhou J, et al.Diversity of functional genes for methanotrophs in sediments associated with gas hydrates and hydrocarbon seeps in the Gulf of Mexico[J].FEMS Microbiology Ecology, 2006, 57: 251-259.[19] Han B, Chen Y, Abell G, et al.Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine[J].FEMS Microbiol Ecol, 2009, 70: 196-207.[20] Krista K, Kristin B, Harala B.Characterization of cultured independent and dependent microbial communities in a high temperature off shore chalk petroleum reservoir[J].Antonie Van Leeuwenhoek, 2009, 96: 423-439.[21] Luz A P, Pellizari V H, Whyte L G, et al.A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil[J].Can J Microbiol, 2004, 50: 323-333.[22] Margesin R, Labbe D, Schinner F, et al.Characterization of hydrocarbon-degrading microbial populations in contaminated and pristine alpine soils[J].Appl Environ Microbiol, 2003, 69: 3085-3092.
